Reaction of azapeptides with human leukocyte elastase and porcine pancreatic elastase. New inhibitors and active site titrants.
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چکیده
منابع مشابه
Is Porcine Pancreatic Elastase a Suitable Model for Human Leukocyte Elastase?
Human Leukocyte Elastase (HLE) has recently been implicated as a poor prognostic marker in certain breast cancers and as a key component involved in Chronic Obstructive Pulmonary Diseases. Because HLE is so expensive, Porcine Pancreatic Elastase (PPE) has been used to model elastase activity and inhibition. This paper elucidates the differences and similarities between HLE and PPE through enzym...
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The reactivity and specificity of a series of substituted benzenesulfonyl fluorides with human leukocyte (HL) elastase, cathepsin G, porcine pancreatic (PP) elastase, and bovine chymotrypsin A alpha are reported. Benzenesulfonyl fluorides with 2-fluoroacyl substituents were found to be potent and specific inhibitors of elastase. HL elastase was inhibited most rapidly by 2--(CF3CF2CONH)--C6H4SO4...
متن کاملHuman leukocyte elastase inhibitors: designed variants of human pancreatic secretory trypsin inhibitor (hPSTI).
Variants of human secretory trypsin inhibitor were constructed with the aim of producing inhibitors specific for human leukocyte elastase. Models of the hPSTI/HLE and hPSTI/chymotrypsin complexes were generated by computer aided protein design and used to plan better HLE inhibitors. This resulted in the production of the strongest and most specific inhibitors of HLE known.
متن کاملThe specificity of purified porcine pancreatic elastase.
An electrophoretically homogeneous elastase preparation free from tryptic and chymotryptic activities was obtained by chromatography on DEAE-Sephadex and CM-cellulose. This preparation exhibits a narrower specificity towards peptide bonds than that observed by Naughton & Sanger (1961). With oxidized insulin B chain as substrate, the fastest breaks occur between alanine-14 and leucine-15 and bet...
متن کاملCleavage of human von Willebrand factor by porcine pancreatic elastase.
von Willebrand factor (vWF) was purified from pooled normal plasma, radiolabeled with iodine then cleaved by porcine pancreatic elastase. Cleavage was monitored by sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE), SDS-agarose electrophoresis, crossed immuno-electrophoresis, ristocetin and botrocetin cofactor activities, and ristocetin and botrocetin induced binding to fixed ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1984
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)43043-2